The Spireites will face Lincoln City and Grimsby Town in the Bristol Street Motors Trophy Group G, which will be completed following the draw on Thursday.
One of the following Premier League U21 teams from the northern section will join the group following the draw, which is live on Sky Sports News on Thursday, June 27, from 2.30pm.
Northern section ball numbers:
1. Aston Villa U21
2. Everton U21
3. Liverpool U21
4. Manchester City U21
5. Manchester United U21
6. Newcastle United U21
7. Nottingham Forest U21
8. Wolverhampton Wanderers U21
Dates and ticket news for these fixtures will be announced in due course.