The Spireites suffered a 4-1 defeat to the hands of Grimsby Town at the Technique Stadium.
The Spireites got off to a great start with an early goal from Laurence Maguire, however they fell away as the game progressed.
The Spireites sit fifth in the Vanarama National League after this result.
Ryan Taylor had the first shot of the game just inside the box but dragged his effort wide.
The Spireites took the lead six minutes into the game. A fantastic Tom Whelan cross from the right-hand side found Maguire in the box, who headed the ball home.
Grimsby should have equalised in the tenth minute; the ball was squared to Jordan Maguire-Drew who managed to put the ball over the bar from two yards out.
Grimsby equalised in the 27th minute through John McAtee,who struck the ball from around 25 yards out and found the bottom right corner.
Gavan Holohan gave Grimsby the lead in the 31st minute with a fantastic strike from 30 yards out that nestled in the top left corner.
The Spireites went into half time a goal behind, with the score 2-1 to Grimsby.
Alex Whittle tried his luck early in the second half, driving towards the Grimsby defence down the left, but his shot from the edge of the box was saved by Max Crocombe.
Grimsby extended their lead in the 50th minute when Harry Clifton met the ball with his head at the back post.
Scott Loach made a great save in a one-on-one situation after Taylor broke away.
Akwasi Asante came close to grabbing a goal back for the Spireites after the ball was laid off to him on the edge of the box, when his strike hit a post.
Taylor added another goal for Grimsby in the 78th minute. The ball was pulled back to him in the box and his strike flew past Loach into the bottom corner.
Liam Mandeville had a chance for the Spireites with a volley from close range, however, Crocombe saved.
Jeff King also tested Crocombe from range, the Grimsby keeper saving comfortably, diving to his left.
The Spireites will look to get back winning ways in next Saturday’s away fixture against Wealdstone.
Spireites: Loach, Whittle, Maguire, Grimes ©, King, Weston, Khan (Quigley 57’), Whelan (Oyeleke 57’), Miller (Rowley 85’), Mandeville, Asante
Unused Subs: Gunning, McCourt
Attendance: 7819 (1718 away)
